|
KMID : 0545119990090040522
|
|
Journal of Microbiology and Biotechnology
1999 Volume.9 No. 4 p.522 ~ p.524
|
|
|
A Method for the Separation of Mouse Pancreatic Islets Using Discontinuous Percoll Gradient Centrifugation
|
|
Cho, Yu Ree
Kim, Sang Duk/Chang, Hyo Ihl/Sung, Ha Chin/Lee, Cherl Ho/Kim, Chan Wha
|
|
|
Abstract
|
|
|
|
A discontinuous Percoll gradient was used to separate islets from the collagenase- treated mouse pancreas easily and rapidly. Since the osmolality of Percoll is very low, adjustment of its osmolality to 340 mOs/㎏ H^2O was essential for securing the optimal separation. A discontinuous gradient layering with Percoll solution of 1.09g, 1.07g, and 1.05g/㎖, respectively, when centrifuged at 800×g for 10 min, resulted in an optimal condition for separation and yielded a banding pattern with an even distribution of islet cells. No significant difference was observed in the morphological features between the Percoll-isolated and the manually-isolated islets. In conclusion, the discontinuous Percoll gradient can be effectively used to isolate the pancreatic islets from mice with four-fold higher efficiency compared to the handpicking method.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|